In vitro comparative evaluation of apical microleakage with three different root-end filling materials

Aim: To compare and evaluate the microleakage of three root end filling materials -Mineral Trioxide Aggregate (MTA), Biodentine and bioaggregate using vacuum dye penetration technique. Materials and methodology: Thirty extracted single rooted premolars were selected and decoronated. Root canals of teeth were cleaned, shaped using hand protaper file and obturated with corresponding gutta percha and AH-plus sealer. The teeth were stored in saline. After 1 week, apical 3mm of the root was resected at an angle of 90° to the long axis of the root. Teeth were divided into three experimental groups of six teeth in each group according to the filling material. The root surfaces were covered with nail varnish up to 2mm from the apical foramen, then immersed in 0.2% Rhodamine B solution in a vaccum chamber and maintained in the dye at 37 C and 100% relative humidity for 24 hours for evaluation of marginal leakage. The teeth were then sectioned longitudinally & examined under stereomicroscope. The depth of dye penetration was measured in millimeters and statistical analysis was carried out. Statistical analysis was carried out using one way ANOVA test. Results: Comparison of microleakage showed maximum peak value of 0.58 mm with a standard deviation of 0.14 for MTA, 0.37mm with a standard deviation of 0.16 for biodentine and 0.22 with a standard deviation of 0.13 for bioaggregate. Results showed p < 0.003, which was statistically significant. Conclusion: Thus it can be concluded that Bioaggregate showed a better result as root end filling material to prevent microleakage, in comparison to biodentine and MTA.